Isolasi dan Identifasi Mikroba Rumen Penghasil Antihistamin “Histamine Methyl Transferase”
Abstrak: The aim of this
research was to find antihistamine ”Histamine Methyl Transferase” of microbe
producers through isolation and testing of their activities on various
substrates. The isolation process found four putative isolates i.e. N2k, N2b,
N3 and N5. Their general characteristics were round, big and formed regular
colonies; regular edge and wavy colonies. Colonies were whitish, shiny and had
low convex elevation. N2k, N2b, and N5 isolates were Gram negative bacteria
whereas N3 was Gram positive. Those isolates were non-motile. Further tests
were conducted to examine the isolate capability to produce catalase and
utilize citric acid as the carbon source to which they showed positive result.
Nitrate reduction test showed positive results for N2b and N5 but not forN2k
and N3. The ability to ferment sugars into acid and gas indicated that all
isolates except N3 were capable of fermenting glucose and lactose. However,
only N2k was capable to produce gas.
Penulis: Ning Iriyanti, Budi
Rustomo, Efka Aris Rimbawanto
Kode Jurnal: jpbiologidd090067
