INDUKSI TUNAS PISANG BARANGAN (Musa acuminata L.) ASAL NIAS UTARA MELALUI KULTUR JARINGAN DENGAN PEMBERIAN 2,4-D DAN KINETIN
Abstract: The research on induction
of banana barangan shoot (Musa acuminata L.) from North Nias through tissue
culture by giving 2,4-D and kinetin was conducted in the Laboratory of Tissue
Culture University of North Sumatera from May until October 2014. The main
objective of this research was to know the explants of banana weevil in apical
and basal position with growth regulator 2,4-D with kinetin which are able to
induce shoots from callus initials. On this research, the treatment tested for
induction of shoots was growth regulators 2,4-D in the concentration 0 mg/L, 1
mg/L, 1,5 mg/L, 2 mg/L and 2,5 mg/L with growth regulators kinetin in the
concentration 0 mg/L, 5 mg/L, 6 mg/L, 7 mg/L and 8 mg/L. The research was
designed using Completely Randomized Design (CRD) two factorial with repetition
of experiment. The results of research showed that the interaction between
concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin was fastest (79 days)
forming the shoot derived from explant basal part. The concentration of 2,5
mg/L 2,4-D with 5 mg/L kinetin produced the average number of shoots formed for
3.00 and average number of highest shoots for 1.50 cm.
Keywords: Musa acuminata,
weevil, initiation, callus, shoot, induction, barangan banana, 2,4-D, kinetin
Penulis: Destarius Zebua, Suci
Rahayu, dan Saleha Hannum
Kode Jurnal: jpbiologidd150335
