UTILIZATION OF POLYCLONAL ANTIBODIES PRODUCED IN LOCAL HORSES (EQUUS CABALLUS) AS A RESOURCE FOR DEVELOPMENT OF ELISA CONJUGATE TO DETECT HEPATITIS B VIRUS (HBV) SURFACE ANTIGENS
Abstract: The aim of this
study was to utilize the antibody produced using Indonesia local horses
(Equuscaballus) to make the conjugate of ELISA kit for detection of hepatitis B
virus (HBV) surface antigen(HBsAg). The polyclonal antibodies were isolated and
purified from local horses immunized repeatedlyusing isolated and purified
HBsAg from Indonesia. The antibodies were conjugated with horseradishperoxidase
by a modified method of Nakane and Kawaoi. The conjugate activities were
performed usingthe principle of ELISA test conducted by the researchers as well
as by independent laboratory.Commercial conjugate for HBsAg ELISA was used as a
comparison study. The results of this studyindicated that the antibody produced
from local horses can be used to make conjugates that werecomparable to
commercial HBsAg ELISA kit.
Author: S.N. Depamede, M.
Sriasih, E. Yulianti
Journal Code: jppeternakangg120068
