THE USE OF MICROSATELLITE MARKERS TO STUDY GENETIC DIVERSITY IN INDONESIAN SHEEP
Abstract: The purpose of this
research was to study genetic diversity in Indonesian sheep population
usingmicrosatellite markers. A total of 18 microsatellite loci have been used
for genotyping Indonesian sheep.Total sheep blood 200 samples were extracted
from garut sheep of fighting and meat types, purbalinggasheep, batur sheep and
jember sheep populations by using a salting out method. Microsatellite loci
datawere analyzed using POPGENE 3.2 software. Based on this study obtained 180
alleles from 17microsatellite loci, while average number of alleles was 6.10
alleles (6 to 18 alleles) from fiveIndonesian sheep populations (garut sheep of
fighting type, garut sheep of meat type, purbalingga sheep,batur sheep and
jember sheep population). The average of observed heterozygosity (Ho) and
expectedheterozygosity (He) values were 0.5749 and 0.6896, respectively, while
the genetic differentiation forinbreeding among population (FIS), within
population (FIT) and average genetic differentiation (FST)were 0.1006, 0.1647
and 0.0712, respectively. Genetic distance and genetic tree showed that
Indonesiansheep population was distinct from garut sheep of fighting and meat
types, purbalingga sheep, batursheep and jember sheep population. Based on this
results were needed a strategy for conservation andbreeding programs in each
Indonesian sheep population.
Author: J. Jakaria, M.S.A.
Zein, S. Sulandari, S. Subandriyo, M. Muladno
Journal Code: jppeternakangg120074
