Free Radical Scavenging and α-Glucosidase Inhibitor Activity of Ethanolic Extract of Mucuna pruriens L.
ABSTRAK: Velvet bean seeds are
ubiquitous but the scientific basis for
its medicinal use especially as antioxidant and antidiabetic remains unknown.
Therefore, a study was designed to evaluate
antioxidant and antidiabetic
activities of the
extract, hexane, ethyl
acetate, butanol and water fractions of velvet bean seeds. In order to evaluate antioxidant activity
of extract and fractions using
quercetin, butylated hydroxyanisole (BHA)
as positive control
and 1,1-diphenyl-2-picryl-hydrazyl (DPPH)
free radical scavenging
activity was determined.
The results showed that
IC50 of DPPH
were 22.17; 152.60;
113.03; 4.93; 9.14
μg/mL respectevily;
quercetin 4.28 and
BHA 22.587 μg/mL.
Antidiabetic activity was
determined by using of
α-glucosidase inhibition assay of
extract, four fractions and glucobay as positive control. The results
showed that antidiabetic IC50 were24.44; 19.55; 8.40; 80.98; 48.97μg/mL respectively
and glucobay as positive control 16.23
μg/mL.
Author: Wahyu Widowati, Hana
Ratnawati, Ch. Retnaningsih, Lindayani,
Udju D. Rusdi, Wahyu Winarno
Journal Code: jpfarmasigg110003
