EXAMINATION OF ACID-FAST BACILLI IN SPUTUM USING MODIFIED LIGHT MICROSCOPE WITH HOMEMADE LIGHT EMITTING DIODE ADDITIONAL ATTACHMENT
Abstract: Typical clinical
symptoms and chest X-ray is a marker of Tuberculosis (TB) sufferers. However,
the diagnosis of TB inadults should be supported by microscopic examination.
Currently, Bacilli microscopic examination of acid-fast bacilli (AFB) in sputum
by Ziehl-Neelsen (ZN) coloring is the most widely used. However, for reasons of
convenience, especially for laboratories with a considerable amount of smear
samples, and due to higher sensitivity compared with ZN staining, the World
Health Organization (WHO) has recommended the use of auramine-O-staining
(fluorochrome staining), which is visualized by light emitting diode (LED)
fluorescence microscopy. The aim of this study was to evaluate the performance
of modified light microscope with homemade LED additional attachment for
examination of AFB in sputum using auramine-O-staining method. We compared the
sensitivity and specificity of 2 kinds of AFB in sputum methods: ZN and
fluorochrome, using culture on Lowenstein-Jensen media as the gold standard.
The results showed auramine-O-staining gives more proportion of positive findings (81%) compared to the ZN method (70%). These
results demonstrated that the sensitivity of auramine-O-staining was higher
than ZN, however it gives more potential false positive results than ZN. The
sensitivity of auramine-O-staining in detecting AFB in sputum was 100% while
the specificity was 88%.
Author: Aryo Tedjo, Anis
Karuniawati, Prawoto, Subari A. Riyanto, Anwar S. Ibrahim
Journal Code: jpkedokterangg110220
