DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR DETERMINATION AND QUANTIFICATION OF RUBRAXANTHONE IN STEM BARK EXTRACT OF MANGOSTEEN
Abstract: Rubraxanthone is one
of antibacterial and antioxidant
compound which is isolated from stem bark of mangosteen (Garcinia mangostana
Linn.). The precise method for analysis of plant constituents is normally a
reverse-phase high performance liquid chromatographic (RP-HPLC). Development
and validation of a RP-HPLC method for chromatographic separation was carried
out on a reversed-phase column Shimadzu ®
Shimp-pack VP – ODS (4.6x250mm) using an isocratic mobile phase of 0.4% formic acid - acetonitrile at a flow rate 1mL min-1, and detection was with a UV detector. The linearity of the proposed method was found in the range of
2.5-25μg/mL-1 with regression coefficient 0.999. Intraday precision studies
showed the relative standard deviation ≤1.58% and inter-day ≤3.20%. Accuracy of the method was
determined by a recovery study conducted at 3 different levels, and the average
recovery was 102.18%. The Limit of Detection Limit of Quantitation (LOQ) and
were 0.47 and 1.56μg/mL-1.The contents of rubraxanthon in the crude was
0.23±0.07%w/w.
Author: Meri Susanti,
Dwisesaria Irma Lena, Dachriyanus Dachriyanus
Journal Code: jpfarmasigg140035
