DETECTION OF MENDELIAN AND GENOTYPE FREQUENCY OF GROWTH HORMONE GENE IN ONGOLE CROSSBRED CATTLE MATED BY THE ARTIFICIAL INSEMINATION TECHNIQUE
Abstract: The objectives of
this study were to detect the Mendelian mode inheritance of growth hormone(GH)
and to establish genotype frequency of GH gene in Ongole-crossbred cattle mated
by the artificialinsemination (AI) technique. Total of 76 blood samples were
collected from Ongole-crossbred cows andbulls (G0), and their progenies (G1) at
the Tumaratas AI service center in North Sulawesi province,Indonesia. All blood
samples were screened for the presence of GH locus using a PCR-RFLP
methodinvolving restricted enzyme Msp1 on 1.2 % of agarose gel. Data were
analyzed using statistical programfunction in Excel XP. The results showed that
GH locus using alleles of Msp1+ and Msp1- enzymerestriction in Ongole-crossbred
cows and bulls was inherited to their Ongole-crossbred progeniesfollowing the
Mendelian mode inheritance. This Mendelian inheritance generated by AI
technique wasnot under genetic equilibrium for the Msp1 genotype frequencies in
groups of G0 and G1. The breedingprogram using genotypes of bulls and cows (G0)
for generating the genotype of GH Msp1 enzymerestriction by AI technique should
be maintained to increase these various allele dispersion rates forbreeding
under genetic equilibrium of the Ongole-crossbred cattle population.
Author: U. Paputungan, L.
Hakim, G. Ciptadi, H.F.N. Lapian
Journal Code: jppeternakangg120064
