Uji in Vitro Penghambatan Aktivitas Escherichia coli dengan Tepung Cacing Tanah (Lumbricus rubellus)
Abstract: This research was
conducted to study the inhibition growth of E. coli by using earthworm
(Lumbricus rubellus) meal. The earthworm meal was used in various
concentrations, i.e. 0, 25, 50, 75 and 100 mg of earthworm meal in 100 ml DMSO
for 0%, 25%, 50%, 75% and 100% (w/v) as treatments respectively. Data were
analyzed by ANOVA in Randomized Complete Block Design. Duncan's multiple range
test and polynomials orthogonal were used. Inhibition effects were measured
through agar well diffusion test. Results showed that earthworm meal contain
antibacterial compound which inhibit E. coli activity. There was a significant
difference (P < 0.05) between earthworm meal treatments and control. The
best antimicrobial effect was found in treatment of 50% (w/v) of earthworm meal
and significantly higher than those of 25, 75 and 100% (w/v), but 25% (w/v) was
not different (P > 0.05) with 75% (w/v). It is concluded that earthworm meal
is capable to inhibit E. coli in-vitro at the optimum level of 50% (w/v).
Penulis: H Julendra, A Sofyan
Kode Jurnal: jppeternakandd070101
