PERILAKU DISOLUSI KETOPROFEN DAN INDOMETASIN FARNESIL TERSALUT GEL KITOSAN-GG
ABSTRACT: Chitosan, a
modification of shrimp-shell waste, has been utilized as microcapsule. However,
it's fragile gel property needs to be strengthened by adding glutaraldehyde
(glu) and natural hydrocolloid guar gum (gg). This research's purposes were to
determine rheological properties of chitosan-guar gum gel, to study diffusion
and dissolution behaviour of ketoprofen and infar through optimum chitosan-guar
gum gel membrane and microcapsule, respectively, and to test the coating
stability of both medicines by the gel microcapsules, which are new drug's
preparation, to determine their shelf lives and to predict the degradation
mechanisms. This research was designed in six (6) steps: (1) chitin isolation
and chitosan synthesis; (2) synthesis and optimization of chitosan-guar gum gel
membrane; (3) in vitro study of ketoprofen and infar diffusion behaviour
through the optimum membrane; (4) synthesis and optimization of chitosan-guar
gum gel microcapsule to coat ketoprofen and infar; (5) in vitro study of
ketoprofen and infar dissolution behaviour from the optimum microcapsule; and
(6) physical and chemical microcapsule stability test using relative humidity
(RH) and temperature controlled climatic chamber method. Studies on ketoprofen
diffusion through chitosan-guar gum membrane showed that the formation of
membrane small pores were appeared to be caused by membrane swelling, which was
supported by the forcing force resulted from the difference of ketoprofen
concentrations in the diffusion cells and from the temperature increase. This
unique pore opening process is excellent for drug delivery process as a
microcapsule. Spray drying process had successfully coated ketoprofen and infar
in chitosan-guar gum microcapsule. Optimization by using Minitab Release 14
software showed that among the microcapsule compositions studied, [gg] and
[glu] of 0.35% (w/v) and 3.75% (v/v), respectively were optimum to coat
ketoprofen, whereas [gg] and [glu] of 0.05% (w/v) and 4.00% (v/v), respectively
were optimum to coat infar, at constant chitosan concentration (1.75% [w/v]).
In vitro dissolution profile showed that chitosan-guar gum gel microcapsule was
more resistant in intestinal pH condition (rather basic) compared with that in
gastric pH (very acidic). From stability test, formulation of ketoprofen
preparation composed of 1.75% (w/v) chitosan, 0.35% (w/v) gg, and 3.50% (v/v)
glu, was relatively the best, ·with ketoprofen percentage left in microcapsule
after 3 months, degradation rate constant, and shelf life of of 80.33%, 0.0351
% week-1 and 18.92 months, respectively. The degradation of ketoprofen was seem
to follow autocatalytic reaction mechanism controlled by the formation and
growth of reaction core. In the other hand, the formulation with composition of
1.75% (w/v) chitosan, 0.19% (w/v) gg, and 5.00% (v/v) glu, was relatively the
best microcapsule, with infar percentage left in microcapsule after 3 months,
degradation rate constant, and shelf life of 77.67%, 0.0008 %-2 week-1 , and
4.28 week or about 30 days, respectively. The degradation of infar was
presumably caused by hydrolysis.
Penulis: Purwantiningsih
Sugita, Achmad Sjahriza, Bambang Srijanto, Budi Arifin
Kode Jurnal: jppertaniandd080118
