PENGEMBANGAN METODE PENANDA GENETIKA MOLEKULER UNTUK LACAK BALAK (STUDI KASUS PADA JATI)
ABSTRACT: Tracking timber on
teak and teak wood products can be conducted using different methods, such as
DNA finger printing, chemical composition of the wood, Near Infra Red spectra
(NIR) and stable isotopes. Samples were collected from wood material and leaves
in Java (9 Forest Management Units district) of Perhutani to determine: i)
pattern of genetic variation within and among populations, ii) to determine the
protocol for DNA extraction from wood, and iii) to study the feasibility of DNA
marker for timber tracking in the field. Results show that: i) genetic
variation of cpDNA (PCR-RFLP) is low, while RAPD variation is moderate, ii)
there are differences in chemical composition of wood among the Forest
Management Units (FMUs) of Perhutani, iii) variation of isotopic carbon and
oxygen in Central and East Java were higher than from of West Java, iv)
absorbtion intensity of NIR in West Java was higher than for Central and East
Java, and iv) testing of DNA marker showed that genetic structure in the forest
site is not significantly different from that in log yards, indicating that the
timber flow is still according to the procedure. RAPD marker also is able to
determine the origin of illegal timber and wood in industry without clear
identity.
Penulis: Iskandar Z. Siregar,
Ulfah J. Siregar, Lina Karlinasari, Tedi Yunanto
Kode Jurnal: jppertaniandd080104
