Karakteristik Semen Segar dan Kualitas Semen Cair Kuda dalam Pengencer Dimitropoulos yang Disuplementasi dengan Fruktosa, Trehalosa dan Rafinosa
Abstract: The objective of the
experiment was to study the characteristics of stallion fresh semen and the
quality of sperm preserved in Dimitropoulos extender (DV) supplemented with
different concentration of fructose, trehalose and raffinose. Semen were
collected using artificial vagina from three stallions. Semen characteristics
and quality were evaluated macro- and microscopically. Prior to extension,
semen were centrifugated at 3000 rpm for 20 minutes. The condensed sperm were
re-suspended in DV supplemented with different types of carbohydrate to meet
the concentration of 200 million spz/ml. All samples were stored at room and
chilled temperature, and were evaluated for motility and viability every 3 h
and 12 h. The results of the experiments indicated that fresh semen
characteristics were fair good; the volume, consistency, motility, live-dead
ratio, concentration (106/ml), total spermatozoa (109/ejaculate) and
abnormality were 29.25±9.33 ml, watery, 7.00±0.12, 67.08±9.08%, 77.89±6.46%,
211.88±21.15, 6.28±2.45 and 27.26±4.64%, respectively. The supplementation of different
type and concentration of carbohydrates did not significantly affect the
motility and viability. However, the supplementation of 50 mM fructose
significantly increased the motility and viability of the sperm compared to the
control. In conclusion, carbohydrate supplementation in DV may not maintain the
sperm quality, particularly in the medium with the osmolarity higher than 400
mOsm/kg.
Penulis: Yudi, I Arifiantini,
B Purwantara, T L Yusuf
Kode Jurnal: jppeternakandd070081
