Identifikasi Genetik Menggunakan Marker Mikrosatelit dan Hubungannya dengan Sifat Kuantitatif pada Sapi
Abstract: The occurrence of
genetic changes in Bali and Brangus cattle crossing and it's relationship to
quantitative traits (e.g. growth and birth weight) were identified using
microsatellite markers. Sixteen microsatellite loci were amplified using
polymerase chain reaction (PCR) technique, the amplified products were then
separated on vertical discontinuous polyacrylamide gel electrophoresis (PAGE)
and silver-staining method was applied to detect the fragment. The evaluation
to the stability and allele inheritance pattern were checked using Chi-Square
segregation analysis. The results of this research showed that allele numbers
average detected in Brangus and Bali cattle and their progeny were 2.31, 2.56,
and 2.75 respectively. At most microsatellite loci, the allele distributions
incline to concentrate and to form bimodal trend. Of 16 micro satellite loci
tested, 5 microsatelitte markers namely INRA 037, HEL 9, CSSM 66, INRA 035 and
ETH 225 indicated a significant response to average daily gain. In terms of
birth weight, a significant response was shown by INRA 037.
Penulis: Maskur, Muladno, B Tappa
Kode Jurnal: jppeternakandd070078
