IDENTIFIKASI BAKTERI Escherichia Coli PENGHASIL PENISILINNASE DARI USUS SAPI, KAMBING DAN AYAM BROILER

ABSTRACT: The aims of this research is to isolate escherichia coli that produced penicilinase. In this  study  15  isolate  of  Escherichia  coli  were  isolated  from  catlles,  goats  and  broiler chickens.  Theh  isolation  and  the  test  were    conducted  base  on  lennette  method.  Sensitivity  test of escherichia coli to ampicillin were conducted using Kirby-Bauer method and the value graded  as  National  Committe  for  Clinical  Laboratory  Standard  (NCCLS).  The  test  for penicillinase were done by using cephalosporin cromogenic disc. The result of sensitivity test shows that 3 isolates of escherichia coli from cattles are sensitive to ampicillin, 2 isolates are intermediate, 1 isolate  frome broiler chicken sensitive to ampicillin, 2 isolates are resistant and to isolates are intermediate. All isolates from goat are resistant to ampicillin. Then, the penicillinase  test  to  escherichia  coli  isolate  that  resistant  to  ampicillin  are  3  isolate  from goats and 2 isolates from broiler chickens are positive produced penicillinase.  
Keyword: escherichia coli, penicillinase, cefalosporine chromogenic
Penulis: Zainuddin
Kode Jurnal: jppertaniandd140352

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