Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP
Abstract: A total of 134
Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain
reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and
allele frequencies of the GH MspI and GH AluI Pesisir cattle were determined.
The GH MspI gene frequencies for the C and T allele were 0.209 and 0.791
respectively, while GH AluI gene frequencies for the L and V allele were 0.992
and 0.008 respectively. The chi-square analysis indicated that this population
is not in Hardy-Weinberg Equilibrium status. Expected heterozygosis value (He)
for GH MspI and GH AluI were 0.3306±0.0266 and 0.0149±0.0073 respectively. The
PCR-RFLP GH MspI marker has higher genetic variability compare to PCR-RFLP AluI
marker. This finding showed that GH MspI T allele was favorable as a GH marker
for Bos indicus breeds.
Penulis: Jakaria, D Duryadi, R
R Noor, B Tappa, H Martojo
Kode Jurnal: jppeternakandd070096
