EFFECT OF MALTOSE CONCENTRATION IN TRIS DILUTION ON EPIDIDYMAL SPERMATOZOA QUALITY OF BALI BULL PRESERVED AT 5 DERAJAT CELCIUS
Abstract: The objective of
research was to evaluate the effect of maltose concentration in Tris dilution
onepididymal spermatozoa quality of Bali bull that preserved at 50C. Five
testis of Bali bull collected fromslaughter house were used in this study.
Epididymal spermatozoa were collected through slicing andflushing methods,
pressing cauda epididymal was conducted in NaCl physiology (NaCl 0.9%)
emulsion.Spermatozoa which collected were divided into three reaction tube and
each diluted by Tris dilutioncontaining: Tris dilution + 20% of yolk (control);
Tris dilution + 20% of yolk + 0.3 g of maltose/100ml(M0.3); and Tris dilution +
20% of yolk + 0.6 g maltose/100 ml (M0.6). Spermatozoa qualities observedwere
motile spermatozoa (MS), live-spermatozoa (LS) and intact-plasma membrane (IPM)
thatevaluated daily in refrigerator at temperature of 5oC. Completely
Randomized Design with threetreatments and five replications was used in this
study. Data was analyzed by analysis of variance.Examination on fresh
spermatozoa showed that spermatozoa concentration was 11,222.5 million
cell/ml,motile spermatozoa was 75.00%, live-sperm was 86.75%, abnormal spermatozoa
was 10.50%,cytoplasmic droplet was 14.00% and IPM was 86.75%. At the seventh
day of preservation, thepercentages of MS, LS and IPM in M0.3 were 37.0 %,
49.2% and 50.4%, respectively, and M0.6 were38.05%; 51.8 % and 52.0%,
respectively that were significantly higher (P<0.05) than control
(29.0%;41.8% and 42.4%, respectively). It was concluded that maltose added into
Tris dilution could lengthenepididymal spermatozoa quality of Bali bull which
persevered at 50C.
Author: J. Wattimena, F.
Parera, M. Veerman
Journal Code: jppeternakangg090016
