Aktivitas antiviral influenza A subtipe H5N1dari ekstrak Brucea javanica L.Merr
ABSTRACT: The outbreak
of influenza A H5N1 subtype
has raised a
global concern on the future
risk of a pandemic.
Oseltamivir, the current
antiinfluenza drug, could
not meet the
demand if there
is a major outbreak. Thus,
there is a need to
find alternative treatment
for influenza A. The
objective of this study was to
determine the antiviral
activity of Brucea
javanica extract against
H5N1 influenza A
virus, inoculated in embryonated chicken eggs. The antiviral activity
was performed in hemagglutination assay. This is a method for tittering
influenza viruses based on their ability to attach to molecules present on the surface
of red blood cells. Serial concentrations of extract using in this study were
tested on embryonated chicken eggs prior to the viral inhibition assay. Result
showed that there is no death of chicken embryos after treated with the maximum
concentration of the extract (1000 µg/ml) incubated for 72 hours. At the same concentration, B.javanica
extract reduced 97.39%
of H5N1 hemagglutinin
titer. In conclusion, B.javanica has
a potential to be developed
as antiinfluenza, especially
for inhibiting the
initial step of viral infection.
Penulis: Neny Purwitasari,
Herra Studiawan
Kode Jurnal: jppertaniandd140334
