RECOVERY OF POLYHYDROXYALKANOATES (PHAs) FROM BACTERIAL CELLS USING ENZYMATIC PROCESS
Abstract: Polyhydroxyalkanoates
(PHAs) are intracellular material accumulated by several bacteria. Commercial
production of PHAs faces the issue of high production cost especially substrate
cost and recovery/separation cost. An alternative to reduce the production cost
is to use enzyme and or chemical to recover PHAs from bacterial cells. Recovery
of PHAs from bacterial cells was done using enzyme, chemical, and a mixture of
enzyme and chemical. Enzyme (s) and or chemical(s) were added into culture
broth to disrupt cells after adjusting pH and temperature of the culture broth.
Treatment by adding enzyme or chemical only into culture broth showed a low
level of PHAs recovered from bacterial cells. Treatment by adding a mixture of
enzymes and chemicals showed the best result among 22 examined combinations,
i.e. a mixture of EDTA, lisozyme, papain enzyme, and SDS. This combination gave
a PHA recovery of 65 % w/w.
Author: S. Marsudi
Journal Code: jpkimiagg060001
