PURIFICATION AND CHARACTERIZATION OF POLY-HYDROXYBUTYRATE (PHB) IN CUPRIAVIDUS NECATOR
ABSTRACT: Purification and
characterization of biodegradable plastic namely Polyhydroxybutyrate (PHB)
in Cupriavidus necator have been carried
out. C. necator
was grown on
a Ramsay medium
with fixed substrate
conditions and optimized for
time. Stepwise purification of PHB was
carried out, by using hydrogen peroxide and chloroform. The effect of
temperature, time, and
hydrogen peroxide concentration
on the purification
were also evaluated.
The extracted PHB was studied
with XRD, FTIR and 1H-NMR and 13C-NMR to
determine its structure and purity. Yield and crystallinity were also studied
with HPLC and XRD, respectively. The results of the research showed that higher
concentrations of hydrogen peroxide gave better yields, whereas higher
temperatures and longer lysis times led to different results.
Higher crystallinity was
observed when purification
temperatures were elevated,
but higher hydrogen peroxide
concentration and longer extraction time gave varying crystallinity. The
highest yield ca 66.10 % DCW was reached by purification using H2O2 20 %, at 100oC for 2 h. The
results of TGA analysis indicated that the
purity of the PHB obtained was about 75 % and by using DSC, it was found that
the PHB showed good thermal properties.
Author: Sergio Leon De Rooy,
Endang Tri Wahyuni, Wiratni, Siti Syamsiah, and Jamil Ismail
Journal Code: jpkimiagg070025
