Pengaruh Pengendapan Protein Serum dengan Pelarut Organik terhadap Profil SDS PAGE Protein Serum
ABSTRACT: A study on the
influence of organic solvent protein precipitation to the profile of the serum
protein has been accomplished. The expected conditions were precipitation of
abundant proteins present in serum result in increasing relative concentration
of minor protein which can be useful for sample preparation for biomarker
studies. The serum were precipitated with various diluted (<10%)
acetonitrile, methanol and ethanol followed by SDS-PAGE analysis of the
supernatant in order to investigate the protein profile. There were no linear
relationship between solvent concentration and the number of protein bands.
However, an optimal condition of precipitation was found which is by methanol
0.1%.
Penulis: Tri Joko Raharjo,
Rusmiati Suprihatin, Deni Pranowo
Kode Jurnal: jpkimiadd070104
