Penentuan Kondisi Optimum Enzim Papain Dari Pepaya Burung Varietas Jawa (Carica papaya)
ABSTRACT: A study to
investigate the optimum condition of papain enzyme has been carried out. The
condition that are investigated are pH and temperature, based on measurement of
enzyme activity which is defined as mmole tyrosin that are released in reaction
between papain enzyme and casein as substrat per minute. In this research, the
papain enzyme was isolated from pepaya burung varietas Java. The enzyme was
partially purified by precipitation method using 30% - 50% saturated acetone.
The result showed that the optimum conditions of papain enzyme are in pH 6 with
activity 2,606 U/mL, and temperature at 50 oC with activity 2,469 U/mL.
Keywords: Papaya var Java,
papain, optimum condition, enzymatic activity
Penulis: Aline Puspita
Kusumadjaja, Rita Puspa Dewi
Kode Jurnal: jpkimiadd050032
