Mikroba Termofil Penghasil Dekstranase dari Nira Panas
ABSTRACT: A thermophilic
aerobe microorganism designated NP4, was isolated from the heated sugar cane.
It grew on dextran, and produced a thermoactive extracellular dextranase.
Screening and isolation was done by assay of dextranase activity semi
quantitatively on solid medium containing blue dextran. It provided several
colonies with different morphology exhibited decolourized zones around, on
culture plates containing blue dextran 2000R. The screening resulted in
isolation of one microbe which efficiently assimilate dextran as carbon source.
Dextranase production from the choised strain in liquid medium was conducted at
room temperature for 8 hours with shaking speed of 125 rpm. The dextranase
enzyme showed optimum pH of 8 and optimum temperature of 60 oC.
Penulis: Afaf Baktir, Zumrotul
Koiriyah, Ali Rohman
Kode Jurnal: jpkimiadd050042
