ISOLATION OF COMMON CARP b-ACTIN PROMOTER
ABSTRACT: Promoter in the
transgene construct plays an important role on regulating of transgene
expression level in transgenic fish. In fish transgenesis, researcher convinced that
use all-fish promoter
more safety and prospective. This study was performed to isolate b-actin
promoter, - the promoter which has ubiquitous, constitutive, house keeping characteristics, from common carp. -actin promoter from common carp (ccBA) was
isolated using PCR method with FBP1, RBP1 and RBP2 primers. Sequencing was
performed using ABI PRISM 3100 machine and analysis of sequences was conducted
using GENETYX version 7 software. The results of sequence analysis showed that the
length of DNA fragment obtained approximately 1,5 kb
and containing the
evolutionary conserved sequences
of transcription factor for β-actin promoter including CCAT, CArG and TATA box. The results demonstrated that isolated
sequence was a common carp b-actin promoter.
Author: Hidayani, A.A, Carman,
O. dan Alimuddin
Journal Code: jpbiologigg130003
