DETERMINATION OF ANNONACYN COMPOUND BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ON THE EXTRACT OF Annona muricata LINN SEED FOR PESTICIDE FORMULA
ABSTRACT: Determination of
annonacyn grade, a main insecticide compound has been done in the ethanolic and
ethyl acetate extracts produced by soxhletation, and ethyl acetate extract
produced by fractionation of ethanolic extract to find out the most active
extract to be used as raw material of pesticide formulae. Analysis method used
the reverse phase high performance liquid chromatography with column of
Novapack ODS C-18 (waters; 3.9 x 150 nm), mobile phase was the mixture of
acetonitrile-water. The result of this research showed the optimum condition as
follows: the mobile phase was acetonitrile-water (60:40), flow rate of 0.2 mL
min -1 , injection volume of 5.0 L, detector UV,
wavelength ( ) at 220 nm with AUFS of 1.00. The limit detection of
annonacyn was 0.01 g, annonacyn grade in ethanolic extract
produced by soxhletation: 0.0405 + 0.0021%, in ethyl acetate extract produced
by soxhletation: 0.0293 + 0.0009%, in ethyl acetate extract produced by
fractionation of ethanolic extract: 0.1003 + 0.0018%. The precision and accuracy
of annonacyn in this research were respectively obtained 8.89% - 1.92%, and
4.91% - 7.28%, at the concentration of 5.00 g mL -1 –
25.00 g mL -1 . The sensitivity was 0.75 < b < 1.13.
Author: Freddy L. Wurangian
Journal Code: jpkimiagg050018
