CAPILLARY ELECTROPHORETIC ANALYSIS OF LOW-MOLECULAR-MASS OF CA SPECIES IN PHLOEM SAP OF Ricinus communis L.
ABSTRACT: A capillary
electrophoretic (CE) analysis with ultra-violet (UV) detection was performed
for further separation of low-molecular-mass (LMM) calcium species in phloem
sap of Ricinus communis L. Two different background electrolytes (BGE) were
used for the separation; these are (1) hydrogen phosphate/dihydrogen phosphate
buffer containing cetyltrimethylammonium bromide (CTAB) as an electro-osmotic
flow (EOF) modifier, and (2) boric acid buffer containing CTAB. Various
parameters affecting the analysis, including the composition and pH of the BGE were
systematically studied. The sensitivity, resolution, baseline noise, migration
time of the species peaks, and reproducibility of the method were evaluated
under optimised condition. At least 13 UV-active species were optimally separated
within about ten minutes. The optimised measurement condition was also achieved
using 10 mM hydrogen phosphate/10 mM dihydrogen phosphate containing 0.5 mM
CTAB at pH 8.0 as BGE, and by applying voltage of -20 kV and temperature of
14°C. Evidently, the analytical method was successfully used for the separation
of LMM calcium species in phloem sap of R. communis L.
Author: Noor Fitri, Björn
Thiele, Klaus Günther and Buchari
Journal Code: jpkimiagg060026
