CALLUS INDUCTION AND PHYTOCHEMICAL CHARACTERIZATION OF Cannabis sativa CELL SUSPENSION CULTURES
ABSTRACT: Callus of Cannabis
sativa has been successfully induced from C. sativa explants and seedings. It
seems that flowers are the best explant for callus induction and induction
under light also give better results than induction in dark. Four cell culture
lines were established from flower induced callus. Phytochemical profiles of C.
sativa suspension cell cultures were investigated using HPLC and 1 H-NMR.
Cannabinoids and phenolic compounds related to cannabinoids such as flavonoids
could not be found in the cell suspension cultures and there is no major chemical
difference between the cell lines though they can visually be distinguished by
their colors. Only in one cell line some aromatic compounds in the
water/methanol extract could be observed in the 1 H-NMR. Further investigations
showed that none of these compounds are flavonoids. It seems that lack of
cannabinoids in the cell cultures is related to lack of polyketide synthase
activity.
Author: Tri J. Raharjo,
Ogbuadike Eucharia, Wen-Te Chang and Robert Verpoorte
Journal Code: jpkimiagg060014
