UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEMANGI (OCIMUM SANCTUM L.) TERHADAP PERTUMBUHAN BAKTERI ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS
ABSTRACT: Escherichia coli and
Staphylococcus aureus are pathogenic bacteria that can cause intestinal and
skin infections. One of the potential medicinal plants that is active against
bacteria is basil leaves (Ocimum sanctum). This research aims to examine the
antibacterial activity of ethanol extracts of basil leaves against the growth
of E. coli and S. aureus. The Research was conducted from September to December
2014. The ethanol extracts of basil leaves in this study used a concentration
of 20%, 40%, 60%, 80%, 100%, and Thiamfenicol as a positive control. The
antibacterial activity test to observe inhibition zone was done through the
disc diffusion method. The results showed that the extracts of basil leaves
were bacteriostatic. In this research, the highest inhibition zone occurred at
a concentrations of 100%, while the optimum treatment in inhibiting the growth
of E. coli and S. aureus was the concentration of 80%. Based on results of the
phytochemical test, the ethanol extract of O. sanctum had a secondary
metabolite compounds i.e. flavonoid, tannin, and essential oil that act as
antibacterial. The Anova statistical results showed a significant difference in
the diameters of the inhibition zone of the treatment of extracts with positive
control.
Penulis: Maria Angelina,
Masnur Turnip, Siti Khotimah
Kode Jurnal: jpbiologidd150118
