DENITRIFICATION BY AZOSPIRILLUM BRASILENSE AND SINORHIZOBIUM SP IN THE PRESENCE OF THE NITRIFICATION INHIBITOR
ABSTRACT: Investigation the
effects of nitrification inhibitor
viz, 3,4dimethylpyrazolephosphate (DMPP
on denitrification and N2O/N2 productions by N-fixers bacteria (Azospirillum brasilense
Az204 and Sinorhizobium sp TNAU14 a model experiment was carried out in
laboratory conditions. As a model experiments consisted of 3 treatments,
control, 1% v/v C2H2 and 1 µgDMPP mL-1
in combination with the inoculation
of Azospirillum brasilense
or Sinorhizobium sp. The concentration
of N2O and CO2, the population density, and the level of nitrate, nitrite, and
ammonium in the medium solution were analyzed. The results showed that the
kinetic of N2O production by Azospirillum brasilense in the presence and
absence of 1 % acetylene ranged about 2–18 µg N2O mL-1day-1, and 2–17 µgN2O
mL-1 day-1. The rate of N2O production by Sinorhizobium sp was about 4–15
µgN2OmL-1 day-1 and 4–12 µgN2OmL- day-1 in the presence and absence of 1%
v/v acetylene.The N2O portion of
total-denitrification los by Azosprillum brasilense or Sinorhizobium sp was about 70–98% and
80–95% and decreased until about 50–51% due to the decreasing of nitrate
supply. The application of 1 µg DMPP mL-1 decreased both N-fixers
population.The CO2-production by Azospirilum brasilense decreased
significantly. DMPP may influence the population of non-target-microorganism in
soils.
Author: Tindaon, F.,
Simarmata, T., Benckiser, G., and Ottow, J.C.G
Journal Code: jpbiologigg110003
