ACTIVITY OF BENZOPHENONE GLUCOSIDE FROM MAHKOTA DEWA {Phaleria macrocarpa (Scheff.) Boerl.} FRUITS ON PROLIFERATION OF HUMAN CERVICAL-CANCER CELLS (HeLa and CasKi) AND HUMAN ESOPHAGEAL CANCER CELLS (TE-2, TE-8, and TE-14)
ABSTRACT: Phaleria macrocarpa,
locally named mahkota dewa, is known as a medicinal plant in Indonesia.
Fruits of this
plant are traditionally
used in treatment
of cancer diseases. Several
substances were isolated
from mahkota dewa
fruits. A benzophenone glucoside,
4,6`-dihydroxy-4`-methoxybenzophenone-2’-O-glucoside, has been
isolated from an
ethyl acetate fraction
of mahkota dewa
fruits. The structure of this
compound was determined based on the analysis of UV, IR, NMR and MS
spectral data. Antiproliferative activity
was measured on
human cervical cancer cells (HeLa
and CasKi) and human esophageal cancer cells (TE-2, TE-8, and TE-14) by MTT
assay. The result of this study showed that at concentration of 500 μg/mL,
benzophenone glucoside derived from mahkota dewa didn’t reach CPI50 to all
tested cells. Those
CPI50 were 34
μg/mL (HeLa), 32
μg/mL (CasKi), 33.91 μg/mL
(TE-2), 35.43 μg/mL
(TE-8), 43.04 μg/mL
(TE-14). There were
no significantly differences (α=0.5) of this activity among HeLa, CasKi,
TE-2, TE-8 and TE-14 cells.
Keywords: Benzophenone
glucoside, Phaleria macrocarpa, HeLa, CasKi, TE-2, TE-8, and TE-14 cells, MTT
assay
Author: A. Diantini, A.
Subarnas, T.H. Achmad, Supriyatna, D. Kurnia, A. Faried, L.S Faried, and H. Hayashi
Journal Code: jpbiologigg080001
