Uji Penghambatan Aktivitas alfa-glukosidase Ekstrak dan Fraksi Daun Antidesma montanum Blume
Abstract: alpha-Glucosidase
inhibitor has known to be a therapeutic agent for diabetes mellitus (DM) treatment,
especially type 2 DM. Based on previous studies. There are various plants that
have the effect of inhibiting the activity of a-glucosidase, one of which is
garu leaves (Antidesma montanum Blume). This research aimed to get the fraction
which had the highest Il-glucosidase inhibiting activity from ethanol extract
of garu leaves and identify the chemical compounds from the most active
fraction. Simplisia powder was extracted by maseration using 80% ethanol then
fractionated using n-hexane, ethyl acetate, and methanol. Inhibitory activity
test was performed by measuring absorbance of p-nitrophenol, which produced by
reaction between Il-glucosidase and p-nitrophenyl-u-l)-glucopyranoside, using
microplate reader at 405 nm. The result showed that ethyl acetate fraction have
the best Il-glucosidase inhibitory activity with IC50 values 138.38 ppm. The
test of enzyme kinetics showed that ethyl acetate fraction inhibited
competitively. The phytochemical screening showed that ethyl acetate fraction
of garu leaves contained glycosides, tannins, and terpenes.
Penulis: Nofiantini
Kode Jurnal: jpfarmasidd120143
