Skrining Fitokimia dan Uji Penghambatan Aktivitas alfa-glukosidase pada Ekstrak Etanol dari Beberapa Tanaman yang Digunakan sebagai Obat Antidiabetes
Abstract: Diabetes mellitus
(DM) is a group of metabolic disorders characterized by hyperglicemia and
associated with abnormalities in carbohydrate, fat, and protein metabolisms.
Patients with DM in the world continue to increase along with population
growths. Starting from this condition, searching for sources of DM treatment is
always performed. One therapy of DM is a-glucosidase inhibitor. a-Glucosidase
is an enzyme that can break down a complex carbohydrate into simple sugar. lhe
inhibiton of this enzyme can retard the rate of carbohydrate digestion
resulting in a delay in glucose absorption. The purpose of this studywas to identify the content of chemical
compound and to test the a-glucosidase inhibitory activity in ethanolic
extracts of some plants used as antidiabetic. a-Glucosidase inhibitory activity
test carried out by spectrophotometric method. The simplisia powder was
extracted by reflux using 80% ethanol. Based on a-glucosidase inhibitory
activity test, all the plant extracts were active in inhibiting a-glucosidase.
lhe three most active extracts were Ceiba pentandra (L.) Gaetern bark extract,
Saccharum offcinarum root extract, and Persea americana Mill. bark extract with
IC50 of 5.16, 10.35 and 10.83 ppm, respectively. They contain glycoside,
tannin, and saponin. The test results of the kinetics enzyme inhibition showed
that Randu bark extract had competitive
inhibitory activity.
Penulis: Abdul Mun’im
Kode Jurnal: jpfarmasidd120050
