PENGARUH SUHU DAN LAMA THAWING DI DATARAN RENDAH TERHADAP KUALITAS SEMEN BEKU SAPI BRAHMAN
ABSTRACT: Thawing is a method
by melting back of semen that had been frozen prior to artificialinsemination
(AI). The temperature and duration of thawing has a major influence on the
state of thespermatozoa, especially the integrity of spermatozoa in the semen.
The combination of temperatureand duration of thawing that will either be able
to prevent damage to sperm , so it still has a highfertility rate. In common,
lowland is an area that has low temperature and humidity, so it will have
aneffect of thawing’s temperature. The purpose of this study was to determine
the temperature andduration of thawing of Brahman’s frozen semen which have the
most optimal to get used inartificial inseminations in lowlands area.Research
was conducted in March 2014 using a completely randomized design (CRD) with
a3x3 factorial. The first factor is the temperature (34°C, 37°C, and 40°C) and
Factor II is duration ofthawing (10 seconds, 15 seconds, and 20 seconds) with 3
replications. Parameters observed in thisstudy is the percentage of sperm
motility and live spermatozoa. The results were analyzed usingANOVA and
Duncan's test at the advanced level of 5 % and 1%. The results showed that
temperatureand duration of thawing give effects to the quality of the frozen
semen of Brahman cattle, but has nointeraction between them. The most good
quality spermatozoa at a temperature of 40ºC spermmotility is 37,77% and the
percentage of live spermatozoa is a 40,83%. Duration of thawing at 15seconds
sperm motility is 38,33% and the percentage of live spermatozoa is a 39,77%.
Penulis: Muhammad Fauzan, Madi
Hartono, Purnama Edy Santosa
Kode Jurnal: jppeternakandd140136
