PENGARUH JARAK STRAW DENGAN NITROGEN CAIR PADA PROSES PRE FREEZING TERHADAP KUALITAS SEMEN BEKU SAPI LIMOUSIN
ABSTRACT: This study aimed to
determine the effect of straw space with liquid nitrogen on pre freezingprocess
and the best straw space to the quality of frozen semen of Limousin Cows so
that they areappropriate for Artificial Insemination process. This study was
conducted in Regional TechnicalService Unit-Regional Artificial Insemination
Office Lampung, Terbanggi Besar District, CentralLampung Regency, Lampung
Province on 7th—13 th April 2014. The Completely RandomizedDesign (CRD) was
used is these research with five treatments of straw spaces, 2 cm, 4 cm, 6 cm,
8cm, and 10 cm, with 4 replications. The data obtained was analyzed by using
variance analysisandcontinued by Polinomial Ortogonal test.The result of this
study showed that the straw space was significantly (P<0,01) to the
motilityand live spermatozoa after thawing. However it was not significantly (P>0,05)
to the motility and livespermatozoa after pre freezing. Polinomial Ortogonal
test, obtained that the straw space wassignificantly (P<0,01) to the
motility and live spermatozoa after thawing, had quadratic pattern withequation
of Y = - 6,50 + 12,09x – 0,89x2 ; R2 = 41,3%, with the best effect on the straw
space of 6 cm,that is 34%. Besides, the straw space was also different
(P<0,01) to percentage of live spermatozoaafter thawing, had quadratic
pattern with equation of Y = 8,97 + 8,81x - 0,64x2 ; R2 = 41,3%, withthe best
effect on the straw space of 6 cm, that is 38,79%.
Penulis: Kurotul Aini, Sri
Suharyati, Madi Hartono
Kode Jurnal: jppeternakandd140145
