IDENTIFIKASI PENYAKIT BAKTERIAL PADA BENIH SIDAT (ANGUILLA MARMORATA) DI BALAI BUDIDAYA AIR TAWAR TATELU
ABSTRACT: This study aimed to
identify the bacterial disease in cultured eel elvers (Anguilla marmorata). The
samples used for this study were taken from the Tatelu Freshwater Aquaculture
Center. The samples taken were elvers (juvenile life stage of A.
marmorata) which showed clinical
symptoms of bacterial infection such as bleeding in the chest, abdomen, and the
base of the fin, passive movement, weakness, and loss of balance of the body,
loss of appetite, some wounds on back fin, chest, and tail, and the body was
not slippery/shiny. Three elvers with the size of 4-6 cm, and weigh about 0.2 g
were taken as samples. The organ chosen to be targeted for isolation was head
kidney. Each sample was isolated twice as replicates and streaked on Triptic
Soy Agar (TSA) medium. First replicates were identified in the Tatelu Health
Lab of Freshwater Aquaculture, and the second replicates were identified in
North Sulawesi Provincial Health Lab. Bacterial identification was done through
a series of observations and morphology of bacterial colonies through gram
stain, followed by biochemical tests through oxidase test, catalase test,
Triple Sugar Iron Agar (TSIA) test, H2S production test, motility test, O/F
test, and Simmons Citrate Agar (SCA) test. Water quality parameters that were
measured included temperature, disolve oxygen, pH, and ammonia. Based on
identification of bacteria in cultured elvers in Tatelu Freshwater Aquaculture
Center, the elver samples had been infected with bacterial Aeromonas hydrophila.
Penulis: Kevin Octavian Kusen,
Reiny A Tumbol, Henky Manoppo
Kode Jurnal: jpperikanandd150026
