Efek Imunostimulan Ekstrak Etanol Kelopak Bunga Rosella
Abstract: The aims of this
study was to determine the immunostimulant potential ethanolic extract of
roselle calyx through the proliferation of lymphocytes and phagocytosis ability
of macrophages Balb/c mice in vitro. Dried calyx of roselle were extracted
using 96% ethanol by maceration method is further polyphenols assay are
qualitatively and quantitatively. Vitro test using with immunostimulants are
seeing an increase in lymphocyte proliferation using the MTT method and
phagocytosis activity macrophages with latex beads. Lymphocyte proliferation is
expressed by the difference in absorbance values between treatment and control
groups, where as the ability of phagocytosis is expressed as an index of
phagocytosis and the SFA. Concentration of extract used was 10-1000 μg/mL.
Ethanolic extract of roselle calyc containing polyphenols with total polyphenol
content (TPC) of 2.757%. Ethanol extract of roselle calyx enhanced immune
response at low concentrations. Concentration
of 100 μg/mL is the optimal concentration that increased proliferation
of lymphocytest. The highest index of phagocytosis and SFA are at
concentrations of 150 μg/mL and 250 μg/mL. It can be concluded that ethanolic
extract of roselle calyx has potential as immunostimulants.
Keyword: roselle, immune response,
macrophage phagocytosis, lymphocyte proliferation, immunostimulant
Penulis: Dita Wulandari, Putri
Ratna Puri, Ratna Kurnia W, Nurkhasanah
Kode Jurnal: jpfarmasidd140108
