DERIVATISASI MORFIN MENGGUNAKAN DANSIL KLORIDA UNTUK MENINGKATKAN KEPEKAAN DETEKSI MORFIN PADA METODE KLT-SPEKTROFOTODENSITOMETER
ABSTRACT: Fluorescence detection
could be used
for enhance the
sensitivity of morphine detection
by TLC-Spectrophotodensytometry.
Morfin is a compound that fluoresces
intrinsically incapable. ence, morphine was
derivatized using dansyl
chloride. Morphine was
derivatized by two
methods, derivatization in solution and overspotting method. The
derivatization reaction was kept at pH 9 and 450 C
for 20 minute.
The ratio between
dansyl chloride and
analyte concentration was
6:1. The derivatization result
was separated from the interference using TLC. The TLC method was using
Al-TLC ilica G60
plate as a
stationary phase and
etyl acetate :
methanol : amonia
(85:10:5) as the mobile phase. The dansyl derivative was
detected at λeks 365 nm and K540 filter for fluoresence.
Derivatization of morphine-dansyl chloride
capable to enhancing
the sensitivity of morphine
detection using TLC-Spectrophotodensytometry. Derivatization in solution and
overspotting method took the
same time in
procedure but they
gave different sensitivity.
Derivatization in solution method
gave more quantity than derivatization
of overspotting.
Penulis: L.P. Ari Chandrayani,
N.P. Linda L., I.N.K. Widjaja
Kode Jurnal: jpfarmasidd120151
