AKTIVITAS ANTIOKSIDAN EKSTRAK UMBI BENGKOANG PADA BERBAGAI UMUR PANEN DENGAN METODE DPPH (2,2-diphenyl-1-picrylhydrazyl)

ABSTRACT: Jicama  or  yam  bean  is  Fabaceae  Family  which  is  potential  as  a  source  of  antioxidants  as  it  is  rich  in  flavonoid  and phytoestrogen. The research was performed in three stages. Stage I: Making of jicama flour with one factor (harvesting age (U): (3, 4, and 5 months). Stage II: Making of jicama methanolic extract with one factor (harvesting age (U): (3, 4, and 5 months). Stage III: Estimating the antioxidant activity of ether/water fractions of methanolic extract with two factors i.e: concentration (K): (10, 20, 40, 80, 160 ppm) and harvesting age (U): (3,4, and 5 months). The results showed that the harvesting  age  had  highly  significant  effect  on  proximate  composition  of  jicama  flour  and  yield  of  methanolic  extract. Moisture content, ash content, and fat content tended to decrease with increasing harvesting age. Meanwhile, crude fiber content tended to increase. The protein content of jicama flour and yield of methanolic extract were maximum at 4 month harvest and tended to decrease at 5 months. The interaction of concentration and harvesting age had highly significant effect on antioxidant activity. The antioxidant of ether fraction tended to increase, but the antioxidant of the water fraction tended to decrease with increasing the harvesting age. The antioxidant of jicama methanolic extract is categorized as a strong antioxidant because the IC50 ether/water fractions were less than 100 ppm.
Keywords: Antioxidant, DPPH, ether fraction, water fraction
Penulis: Juni Annida Lintang, Herla Rusmarilin, Linda Masniary Lubis
Kode Jurnal: jppertaniandd140225

Artikel Terkait :