PRODUKSI BIBIT KELAPA KOPYOR TRUE-TO-TYPE MELALUI TEKNIK KULTUR EMBRYO
Abstract: Kopyor is one of the
most valuable germplasm in Indonesia. However, the production of true-to-type
seedlings with 100 % kopyor nuts is still limited because the nut would not
germinated naturally. The only technique available to solve the problem is by
using embryo culture technique. However, the technique is limited especially on
the low of success rate after acclimatisation step (less than 40 %). Therefore,
we report a new breakthough technique for producing kopyor seedling using
embryo culture technique especially on acclimatisation step. Our results showed
that the best acclimatisation step could be done by culturing 4-month old
seedlings in a culture room with 14 hour light with intensity around 1400 lux
and 10 hours in the dark. Seedlings were cultured for three months in mini
growth chamber with 95 % relative humidity level and temperature around 26 to
28 0C. The survival rate after acclimatisation step on the seedlings with full
root was more than 95 %, while for the seedlings without root was c.a 90 %. The
acclimatized seedlings then underwent to screenhouse for 1 year befor being
field planting. Up to now, more than 170 seedlings of Kopyor have been transfer
to the field for developing seed garden of Kopyor in Purwokerto, Indonesia. In
the future, creating a breeding program to produce hybrid of Kopyor will be
useful for poverty reduction programmes in Indonesia.
Penulis: Sisunandar
Kode Jurnal: jpbiologidd140119
