OPTIMASI UMUR KALUS SEBAGAI DONOR SEL TERHADAP BIOMASSA DAN KADAR SAPONIN PADA KULTUR AGREGAT SEL Talinum paniculatum (Jacq) Gaertn
Abstract: This study was aimed
to explore the age appropriate of callus which forming cell suspension in
liquid culture. T. paniculatum leaf explants planted on solid MS medium with
the addition of 2 ppm of 2,4 D and 1 ppm kinetin. Callus was on 3, 4, 5, and 6
weeks moved separately in 50 mL of liquid MS with 1 ppm 2,4 D and 1 ppm
kinetin, agitated at 100 rpm for six days in absolute dark conditions (25 ±
1oC). Dry biomass and total saponins were measured by the Hu method. Callus on
4-week-old callus has the highest biomass (0.16 x 10-3 g), but the highest
levels of saponins derived callus at the age of 5 weeks (17.45 ppm).
Penulis: Agus Muji Santoso
Kode Jurnal: jpbiologidd140117
