Optimasi Komposisi Fasa Gerak dan pH Buffer Asetat Pada Analisa Zat Warna Sintetik Rhodamin B dan Ponceau 4R Menggunakan Metoda HPLC
Abstract: One of the food
addictives that is often used by many people is dye. More synthetic dyes is
used in food and beverage than natural dyes time by time. According to
Regulation of Health Ministry No.239/Menkes/Per/VI/85, Rhodhamin B is synthetic
dye which is banned because it is a carcinogen.
However, Ponceau 4R is allowable synthetic dye. The way of using Ponceau
4R must be based on regulation of the Indonesia Health Ministry No.
722/Menkes/per/IX/88; 70 mg/kg for beverage and 300 mg/kg for food. The purpose
of this research is to find the optimum condition of the mobile phase
composition and pH buffer acetate. The
analysis of the Rhodamin B and Ponceau 4R were done by optimalizing the mobile
phase composition and pH acetate buffer with methanol mobile phase: acetate
buffer and stationary phase ODS C18 column used the HPLC method of the UV-Vis
detector. The result of the optimization pH acetate buffer was pH 6 acetate
buffer with a ratio composition of methanol: buffer (80:20) with a wavelength
of 540 nm. Retention times obtained for Rhodamine B was 5,048 minutes and
Ponceau 4R was 2,528 minutes. There were four samples containing dye Ponceau 4R
and none containing dye Rhodamine B were found by using the HPLC method from
the ten samples tested
Penulis: Liza Olivia, Budhi
Oktavia, Iryani Iryani
Kode Jurnal: jpkimiadd130527
