OPTIMALISASI ISOLASI DAN PURIFIKASI DNA Petunia hybrida SERI ROSE PICOTEDENGAN KIT ISOLASI GENEAID
Abstract: Petunia has been
used as research object on flavonoids studies especially anthocyanin
biosynthesis. Hf1 gene encode F3'5'H enzyme which plays a role in the synthesis
of color in Petunia. Research on the regulation of Petunia color is potential
for the development of ornamental plant breeding in Indonesia, hence the
information regarding the gene analysis is necessary. Recently, there are many
reagent kits for the isolation and purification of plant DNA available, each
has different protocols regarding the special character of plant tissue with
various secondary metabolites accumulation. Secondary metabolites in plants have
different chemical contents, for that reason its needs to develop a proper
protocol resulting in pure DNA with high concentrations. This study aims to
obtain the effective and efficient DNA isolation and purification protocol for
Petunia hybrida. Optimization results showed that samples immersed in absolute
ethanol for two days then dried and stored in the freezer until used have given
the optimal results for further analysis. The total DNA concentration was 129.3
ng / µl with purity of 1,87.
Penulis: Naailatu Nur Azizah
Kode Jurnal: jpbiologidd140152
