DEPOLIMERISASI KITOSAN DENGAN HIDROLISA ENZIMATIK MENGGUNAKAN ENZIM Α-AMILASE
ABSTRACT: Chitosan is a
polymer derivative of chitin that has many benefits in food and medical. Since
chitosan has a large molecular weight, it has constraints to its application.
To overcome the disadvantage, it needs hydrolysis of chitosan in order to
obtained a lower molecular weight. In this research the hydrolysis of chitosan
will be operated by enzymatic process
uses thermophilic enzyme α-amylase. The experiment begins by making a chitosan
solution 1% w/v in an acetic acid solution 1% v/v. The chitosan solution is
hydrolyzed using the enzyme α-amylase with varied pH, operating temperature,
time, and ratio of the enzyme:substrate to obtain the optimum. The response
comprehends Dextrose Equivalent (DE), viscosity, and the average of molecular
weight. The results showed that the optimum operating conditions for chitosan
hydrolysis using α-amylase enzyme is at pH 5, temperature 90 °C, 2 hours
operating time, and ratio of enzyme:substrate 1:2500 (0,1 ml). For variable pH,
temperature, time, and enzyme addition, DE rises meanwhile variables are
increased, then it dropped significantly after the optimum level. The
hydrolysis can reduce molecular weight of chitosan from 1680-1750 kDa to 144,18
kDa thus it is included to MMWCs.
Penulis: Hanik Handayani P.L.,
Paramarta Siwi R., Nur Rokhati
Kode Jurnal: jpkimiadd130324
