ASIDOLISIS ENZIMATIK MINYAK IKAN TUNA ( THUNNUS THYNNUS) MENJADI PRODUK ASAM LEMAK KAYA OMEGA-3 DENGAN PEMANFAATAN LIPASE GETAH PEPAYA (carica papaya latex)
ABSTRACT: Incorporation of
omega-3 polyunsaturated acids (n-3 PUFA) into Tuna (Thunnus thynnus) fish oil
was investigated by using acidolysis enzimatis Process. The product of this modification
is expected to be used as an ingredient nutrifition food products, especially
in milk and baby food as well as pregnant and lactating
The purpose of this study was to optimizing the used of lipased papaya
latex (Carica papaya latex) in the product to incorporation fatty acids rich in
omega-3.Bioreactor acidolysis enzematik development and Optimization of process
the productivity of fatty acids rich in omega-3. Reaction Acidolysis was
conducted between tuna (thunnus thynnus) fish oil by microbial lipase (5% of
the weight of the substrate mixture) or vegetable lipased (Carica papaya latex)
(6-10% by weight subtract mixture) as biocatalist.Acidolysis mixture the
bioreactor at 40 o C for (2-6 hours) and the stirring speed of 200 rpm, pH (4.5
to 6.5). to eliminate free fatty acids from the product acidolysis performed
neutralization with NaOH
The results are expected to show that the lipase papaya latex (Carica
papaya latex) can be used as biocatalyst incorporated omega-3 fatty acids
in tunaIthunnus thunnyl) fish oil . In
this study sought conditions for optimum incorporation rate, ie the use of
papaya latex lipase concentration of 8%, the ratio of concentrations of omega-3
fatty acid and palm oil (1:1), pH = 5.5, Optimal time of 4 hours and the temperature
(40oC) . The results were analyzed incorporation of omega-3 (EPA and DHA) with
GC.EPA generated: 3.57% and DHA = 3.91%
Penulis: Wahyuningsih, Isti Pudjihastuti, Heny
Kusumayanti
Kode Jurnal: jpkimiadd120241
