PRODUCTION OF BIOETHANOL FROM CITRUS FRUIT (Citrus sp) WASTE BY ACID HYDROLYSIS AND FERMENTATION USING Saccharomyces cerevisiae
ABSTRAK: One of the promising
biofuel is bioethanol which can be produced from agricultural waste cellulosic
biomass such as citrus fruit waste. Bioethanol can be derived from citrus fruit
waste that is rarely used. Cellulosecontained in citrus fruit waste can be converted into ethanol through
a process of chemical and biological. Sulfuric acid ( H2SO4) can be used in
hydrolysis of cellulose material in citrus fruit waste to producesugars and
batch fermentation by Saccharomyces cereviseae can be used to convert sugars
into bioethanol.The purpose of this research were to determine the highest
reducing sugar from acid hydrolisis with different acid concentration levels of
0, 3, 7, and 10% and to know the highest levels of bioethanol and optimum
incubation time. Extracts made
from citrus fruit
waste without peel.
Citrus fruits were
hydrolyse using a
variation of 1
M H2SO4 acid concentration of
0, 3, 7,
and 10 % to
produce reducing sugars. Reducing
sugars were analyzed
using the Nelson
Somogyi method.The optimum reducing sugar is used for fermentation by yeast Saccharomyces cerevisiae using a variation of the long incubation days
0, 2, 4, 6, 8 and bioethanol purified by distillation method. Acid hydrolysis resulted reduction sugar
3,5%, at an optimum concentration of 3% is used for fermentation by yeast S.
cerevisiae. The highest level of fermentation time of 6 days produced
bioethanol having the content 9,75% .
Penulis: itra Andini, Edwi
Mahajoeno, Ratna Setyaningsih
Kode Jurnal: jpbiologidd130285
