The Difference of Epidermal Growth Factor Concentration Between Fresh and Freeze-Dried Amniotic Membranes
ABSTRACT: This research has
been conducted to measure the difference of Epidermal Growth Factor (EGF)
concentration between fresh and freeze-dried amniotic membranes. Experimental
one group pretest - post test design was employed with 13 amniotic membranes. Each
amniotic membrane was divided into two parts. The first part was without
preservation (fresh) and the second part was with preservation (freeze-dried).
Both parts of amniotic membrane were extracted using ultrasonic disintegrator
then the concentration of EGF was measured using ELISA method. The result
showed the average concentration of EGF in fresh amniotic membrane was 122,756 ±
11,591 pg/g while in freeze-dried amniotic membrane was 100,436 ± 9,690 pg/g.
Average degradation of EGF concentration was 22,320 ± 15,353 pg/g with
estimated percentage degradation between 11,04–23,96%. It can be concluded that
EGF concentration in fresh amniotic membrane is significantly higher than EGF
concentration in freeze-dried amniotic membrane.
Penulis: Ihsan, Prijanto
Kode Jurnal: jpkedokterandd080010
