The Difference between Total and Active Transforming Growth Factor E2 Concentration on Fresh and Freeze-Dried Amnion Membrane (Laboratory Experimental Study in Dr. Soetomo General Hospital Surabaya)
ABSTRACT: The aim of this
study was to evaluate the change of Transforming Growth FactorE2 (TGF-E2)
concentration due to freeze-dried method. This study was an experimental laboratory
study, performed on 14 amniotic membranes at biomaterial centre/tissue banking Dr.
Soetomo General Hospital Surabaya. Each amniotic membrane was divided into two
parts. The first part was extracted in fresh and the second part underwent
freeze-dried preparation before extracted. Both parts of the amniotic membrane
were extracted using ultrasonic disintegrator. Concentration of TGF-E2 with and
without activation was measured using ELISA method. The average concentration
of TGF-E2 without activation (active) in fresh amniotic membrane was 274 ±
264,9 pg/g and freeze-dried was 76,1 ± 97,6 pg/g, the average degradation of
TGF-E2 was 43,2%. The average concentration of TGF-E2 with activation (total)
in fresh amniotic membrane was 445 ± 855,6 pg/g and freeze-dried was 347,4 ±
665pg/g. Active TGFE2 concentration in fresh amniotic membrane is significantly
higher than TGF-E2 concentration in freeze-dried amniotic membrane.
Keywords: freeze-dried
amniotic membrane, TGFE2, ELISA
PENULIS: Elisabeth Srisubekti,
Diany Yogiantoro, Gatut Suhendro
Kode Jurnal: jpkedokterandd090006
