Perbandingan Efek Ekstrak Eter dengan Ekstrak Etanol Biji Tua Pisang Kluthuk (Musa balbisiana Colla) pada Sekresi Asam Lambung Tikus Putih in vitro
Abstract: Kluthuk banana (musa
balbisiana colla), a seeded banana, has been studied for its ability to reduce
gastric acid secretion. Is the active compound hydrophylic or hydrophobic has
not been known yet.The aim of his study is todetermine the effect of the seed
of ripe kluthuk banana (musa balbisiana colla) etheric and ethanolic extract,
and to compare the effect of both extract on rat gastric acid secretion in
vitro. This study was conducted using isolated
Wistar rat stomach, according to
the methods modified from Barocelli. Fifty four rats (3-4 month) of 150-250 g
wereused in this study. They were divided into 9 groups (6 rats each). The rats
were fasted and drinking water was given adlibitum for 24 hours before testing.
The rats were weighted, anesthetized with ether inhalation, and then sacrified.
The stomach was taken and suspended in an organ bath containing 37°C buffered
serosal solution and bubblewith carbogen. The gastric lumen was perfused
continuously with unbuffered mucosal solution 1 ml min-1 and bubbled with 100%
O2. The isolated preparation was stabilized for 1 hour and perfusate spilled
out. The perfusate + was allowed to flow continuously, and collected for 10
minutes duration. The H consentration was measured by + titration with NaOH
0,002 N using phenolftalein as indicator, known as basal H consentration .
Pretreatment was added to the unbuffered mucosal solution for 30 minutes, after
which the treatment (histamine 10-6 M) was added to the unbuffered solution in
each group. Perfusate from gastric lumen were collected every 10 minutes untill
80 + + minutes and H consentration were measured by mean of titration. The H
consentration elevation was expressed as mean ± SEM, the AUC0-80 was calculated
and analyzed by ANOVA.The result showed that the total area under + curve
(AUC0-80) of H consentration was 14550 ± 692,70 in control group (saline),
141,67 ± 2838,62 in etheric extract of the seed of ripe kluthuk banana doses-1
group (EESRKB I group) equivalent to 1,92 mg/kgBW, 8516,67 ± 3659,64 in EESRKB
II group (equivalent to 3,84 mg/kgBW), 5650 ± 3191,94 in EESRKB III group
(equivalent to 7,69 mg/kgBW), 4500 ± 2819,22 in ethanolic extract of the seed
of ripe kluthuk banana doses-1 group (AESRKB I group) equivalent to 8,7
mg/kgBW, -5883,33 ± 760,45 in AESRKB II group (equivalent to 17,4 mg/kgBW),
-1116,67 ± 3444,36 in AESRKB III group (equivalent to 34,8 mg/kgBW), -1333,33 ±
80,28 in cimetidin group, and 12683,33 ± 968,65 inDMSO group. The statistical
analysis showed that the AUC0-80 of EESRKB and AESRKB were significantly
(p<0,05) lower than those of control solution (except EESRKB II), the
AUC0-80 of EESRKB I were not significantly different (p>0,05) than those of
AESRKB I, the AUC0-80 of EESRKB II were significantly higher (p<0,05) than
those of AESRKB II, and the AUC0-80 of EESRKB III were not significantly
different (p>0,05) than those of AESRKB III. It was concluded that the
etheric and ethanolic extract of the seed of ripe kluthuk banana (Musa
balbisiana Colla) showed an inhibitory effect on rat gastric acid secretion
induced by histamine, and the ethanolic extract showed an inhibitory effect
more than those of etheric extract.
Penulis: Fitria Widyasari,
Domas
Kode Jurnal: jpkedokterandd090046
