Pengaruh Lama Paparan Androgen terhadap Indeks Resistensi Insulin dan Kadar Asam Lemak Bebas pada Serum Tikus Model Sindroma Ovarium Polikistik (Penelitian Eksperimental dengan Rattus norvegicus sebagai Hewan Coba)
ABSTRACT: The objective of
this study was to confirm the increase in insulin resistance index and free
fatty acid concentration after administration in different duration of
testosterone propionate and placebo in Rattus Novergicus strain wistar as an
PCOS model. This study was performed at animal test facility and Pathology
Clinic Department in Veterinary Faculty, Airlangga University, November 2007
until January 2008 period, as approved by Animal Research ethics committee,
Veterinary Faculty, Airlangga University. This was an experimental research
with randomized post test only control group design. Sample are consecutively
recruited based on inclusive criteria and divided into two groups. The
interventional group were stimulated with testosterone propionate for 14, 21
and 28 days. Concentration of glucose were measured by glucose oxydase method,
meanwhile insulin and free fatty acid were measured by ELISA method. Insulin
resistance index was measured with HOMA. There were significant increases in
insulin resistance index between interventional and control groups.
Statistically insulin resistance index on serum rat interventional group is
significantly increase from control group in 14 days administration (3.7933 ±
0.5416 : 0.1800 ± 3.5350, p< 0.05), in 21 days administration (7.4950 ± 2.5289 : 13.2017 ± 4.6473, p< 0.05), and
in 28 days administration (8.2950 ± 2.5403 : 20.1100 ± 9.8162, p< 0.05).
Statistically free fatty acid concentration on serum rat interventional group
is significantly increase from control group in 21 days administration (0.4467
± 0.1199 : 1.1033 ± 1.6224, p< 0.05), and in 28 days administration (0.545 ±
0.2471 : 2.265 ± 1.7539, p< 0,05) but not in group 14 days administration.
In conclusion, insulin resistance index on serum rat interventional groups is
significant increase from control group. Free fatty acid concentration on serum
rat interventional group is significant increase from control group in 21 and
28 days administration.
Keywords: PCOS, insulin
resistance index, SOPK, Testosterone propionate, insulin, glukose, free fatty
acid, HOMA insulin resistance
Penulis: Dwinanto Ananda
Muttaqin, Budi Santoso, Widjiati
Kode Jurnal: jpkedokterandd090002
