Efek Antimikroba Minyak Jintan Hitam (Nigella Sativa) terhadap Pertumbuhan Escherichia Coli In Vitro
Abstract: Black cumin (Nigella
sativa) oil contains substances: thymoquinone, dithymoquinone,
thymohydroquinone, thymol, and tannin which are considered can inhibit the
growth of bacteria, fungi, and parasites, so has an antimicrobial effect. This
research is aimed to know the antimicrobial effect of black cumin oil in
inhibiting Escherichia coli's growth.This is a laboratoric experimental
research using non random consecutive sampling, with local isolate Escherichia coli from Microbiology Laboratory of RSUD Moewardi
Surakarta as the subject of the research. Escherichia coli aged 24 hours on a nutrient
agar and had standardized with Mc Farland 0,5, spread with sterile rib cotton
over Mueller-Hinton gell. An empty filter paper disc as a negative control, 25
µg cotrimoxazol antibiotic disc as a positive control, and a filter paper disc
filled with black cumin oil, are placed on the Mueller-Hinton gell plate. The
plate is incubated for 1 x 24 hours, then the inhibiting zones is
measured. The attempted data were
analized with Kruskal-Wallis test. Result of the research shows that in Escherichia
coli was formed an inhibiting zone. Kruskal-Wallis analysis shows a significant
difference (p < 0,05) between positive control (cotrimoxazol 25 µg) with
black cumin oil 50% (8,80 ± 0,75 mm), 75% (10,05 ±1,14 mm), and 100% (12,05±0,83
mm) in concentration. Whereas black cumin oil 25% (0,00 ± 0,00 mm) in
concentration and negative control (0,00 ± 0,00 mm) don't show a significant
difference (p > 0,05). The
conclusion of the research is black cumin (Nigella sativa) oil has an
antimicrobial effect to Escherichia coli's growth.
Penulis: Asniyah
Kode Jurnal: jpkedokterandd090057
