Deteksi Mycobacterium tuberculosis dengan Teknik PCR pada Cairan Efusi Pleura Penderita Tuberkulosis Paru
Abstract: Tuberculosis pleural
effusion is a manifestation of
extrapulmonal tuberculosis, which is rampant in various countries
in the world, including Indonesia.
Nowadays one-third of the world population has been infected by tuberculosis.
Every year there are approximately 8 million new tuberculosis patients
throughout the world and nearly 3 million people die of this disease every
year. Pathogenesis of tuberculosis pleural effusion is a pleural
hypersensitivity reaction accompanied by a small number of Mycobacterium tuberculosis. The objective of
this research was to detect
Mycobacterium tuberculosis in pleural effusion liquid by means of PCR
technique to establish diagnosis of tuberculosis pleural effusion. On this
research, detection of Mycobacterium tuberculosis by PCR technique was compared
with microscopic examination by means of Ziehl-Neelsen (ZN) method. The
research result showed that 9 out of 11 samples were positive by using PCR
technique whereas with ZN method all samples showed negative result. The conclusion
of this research was that Mycobacterium tuberculosis in small numbers could be
detected in pleural effusion liquid by means of PCR technique so that diagnosis
could certainly be established.
Penulis: Diana Krisanti
Jasaputra, Jahja Teguh Widjaja, Teresa Liliana Wargasetia, Iryanthy Makangiras
Kode Jurnal: jpkedokterandd0700156
